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Dalmasso G, Charrier-Hisamuddin L, Nguyen HT, et al

An average brand may contain about 25 mcg, which is more than 400% of the recommended daily value

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Purification of the crude by HPLC (eluent A: 0.1% TFA in water, eluent B: 0.1% TFA in acetonitrile/water 4/1 v/v, 50 C, detection at 215 nm, 3 mL min 1 , 010% eluent B in 5 min, then 1035% eluent B in 35 min, then 3550% eluent B in 15 min, C18 column) provided RLKEPVHGA-SetCys-ALKEPVHGV-NH 2 as a white solid after lyophilization (15.1 mg, 41%)